Journal: Aging

Article Title: Comprehensive analysis of transcriptome data and experimental identification show that solute carrier 35 member A2 (SLC35A2) is a prognostic marker of colorectal cancer.

doi: 10.18632/aging.205145

Figure Lengend Snippet: Figure 6. GO/KEGG analysis of gene SLC35A2 in colorectal carcinoma. (A) GO analysis of SLC35A2. The results were classified into Biological Process (BP), Cell Composition (CC) and Biological Function (MF). (B) KEGG analysis of SLC35A2 in colorectal tissues.

Article Snippet: PBS buffer was used for 3 × 3 min, endogenous peroxidase was dripped into tissues for incubation for 10 min, the PBS buffer was used for 3 × 3 min, and SLC35A2 (13657-1-AP, Proteintech Group Inc., IL, USA) was diluted to 1:100.

Techniques:

Journal: Aging

Article Title: Comprehensive analysis of transcriptome data and experimental identification show that solute carrier 35 member A2 (SLC35A2) is a prognostic marker of colorectal cancer.

doi: 10.18632/aging.205145

Figure Lengend Snippet: Figure 7. Construction of a nomogram to predict prognosis of patients with colorectal cancer. (A) Based on SLC35A2 expression and clinical characteristics of patients “TCGA-AA-A02K”, a nomogram was constructed. The 1, 3 and 5-years mortality rates of patient “TCGA-AA- A02K” were 0.292, 0.587 and 0.839, respectively. (B–D) The AUC of this nomogram with 1, 3 and 5-year ROC curves were 0.797, 0.8 and 0.818, respectively. (E–G) Calibration curves of 1, 3, and 5 years indicated that the nomogram was able to accurately predict colorectal cancer patients’ survival.

Article Snippet: PBS buffer was used for 3 × 3 min, endogenous peroxidase was dripped into tissues for incubation for 10 min, the PBS buffer was used for 3 × 3 min, and SLC35A2 (13657-1-AP, Proteintech Group Inc., IL, USA) was diluted to 1:100.

Techniques: Expressing, Construct

Journal: Cell & Bioscience

Article Title: Activation of goblet cell Piezo1 alleviates mucus barrier damage in mice exposed to WAS by inhibiting H3K9me3 modification

doi: 10.1186/s13578-023-00952-5

Figure Lengend Snippet: Impaired mucus barrier function in the WAS model mice. A , B Pain threshold and AWR score in the control and WAS mice (n ≥ 6). C , D Colon mucus thickness and increased mucus thickness in the control and WAS mice measured by horizontal ussing chamber (n ≥ 6). E RT-PCR of mucin2 mRNA in the mucosa of control and WAS mice (n ≥ 6). F The ratio of goblet cells to epithelial cells in the control and WAS mice (n ≥ 6). G Immunofluorescence of mucin2 in the control and WAS mice; bar length: 50 μm. H Alcian staining of control and WAS mice colon; bar length: 50 μm. I FISH of control and WAS mice colon, mucus was stained with UEA1 (green), bacteria were detected by fluorescence in situ hybridization with general bacterial 16S probes EUB (red) and DNA stained using DAPI (blue); bar length: 50 μm and 20 μm

Article Snippet: As in previous research [ ], paraffin-embedded Carnoy’s-fixed sections were hybridized with 10 ng/ml of a general bacterial 16S rRNA probe (EUB338, red, 5′-GCT GCC TCC CGT AGG AGT-3′, Boster) and immunostained for Mucin2 using the UEA-1 (Bauhinia bean lectin, FL-1061-2, Vector labs).

Techniques: Control, Reverse Transcription Polymerase Chain Reaction, Immunofluorescence, Staining, Bacteria, Fluorescence, In Situ Hybridization

Journal: Cell & Bioscience

Article Title: Activation of goblet cell Piezo1 alleviates mucus barrier damage in mice exposed to WAS by inhibiting H3K9me3 modification

doi: 10.1186/s13578-023-00952-5

Figure Lengend Snippet: Piezo1 agonist improved mucus barrier function and ameliorated intestinal motility disorder in WAS mice. A , B Pain threshold and AWR score in the control, WAS and WAS + Yoda1 mice (n ≥ 5). C , D Colon mucus thickness and increased mucus thickness in the control, WAS and WAS + Yoda1 mice measured by horizontal Ussing chamber (n ≥ 5). E Alcian staining of control, WAS and WAS + Yoda1 mice colon; bar length: 50 μm. F The ratio of goblet cells to epithelial cells in the control, WAS and WAS + Yoda1 mice (n ≥ 6). G RT-PCR of mucin2 mRNA in the mucosa of control, WAS and WAS + Yoda1 mice (n ≥ 5). H FISH of control, WAS and WAS + Yoda1 mice colon; mucus was stained with UEA1 (green), bacteria were detected by fluorescence in situ hybridization with general bacterial 16S probes EUB (red) and DNA stained using DAPI (blue); bar length: 50 μm and 20 μm. I Results of Ink propulsion test in the control, WAS and WAS + Yoda1 mice. J – L Spontaneous activities and Acetylcholine (Ach) induced responses of colonic longitudinal muscles, including MI and MI compared to baseline, in the control, WAS and WAS + Yoda1 mice (n ≥ 5)

Article Snippet: As in previous research [ ], paraffin-embedded Carnoy’s-fixed sections were hybridized with 10 ng/ml of a general bacterial 16S rRNA probe (EUB338, red, 5′-GCT GCC TCC CGT AGG AGT-3′, Boster) and immunostained for Mucin2 using the UEA-1 (Bauhinia bean lectin, FL-1061-2, Vector labs).

Techniques: Control, Staining, Reverse Transcription Polymerase Chain Reaction, Bacteria, Fluorescence, In Situ Hybridization, Muscles

Journal: Cell & Bioscience

Article Title: Activation of goblet cell Piezo1 alleviates mucus barrier damage in mice exposed to WAS by inhibiting H3K9me3 modification

doi: 10.1186/s13578-023-00952-5

Figure Lengend Snippet: Decreased mucus expression and increased methylation in the Piezo1 flox-mucin2 Cre mice. A Immunohistochemistry of Piezo1 in WT and Piezo1 flox-mucin2 Cre mice (KO mice); bar length: 20 μm. B Western blot of Piezo1 in WT and KO mice. C , D Alcian staining and statistical analysis of mucus thickness in WT and KO mice (n ≥ 6); bar length: 50 μm. E RT-PCR of mucin2 in WT and KO mice (n ≥ 6). F , G Immunofluorescence and statistical analysis of goblet cells/ epithelial cells in WT and KO mice (n ≥ 6); bar length: 20 μm. H FISH of WT and KO mice, mucus was stained with UEA1 (green), bacteria were detected by fluorescence in situ hybridization with general bacterial 16S probes EUB (red) and DNA stained using DAPI (blue); bar length: 20 μm. I – J Western blot and statistical analysis of SUV39h1, Hdac3 and H3K9me3 in the colon mucosa of WT and KO mice. K Immunofluorescence for co-localization of AGR2 (label of goblet cells) and Piezo1 in intestinal mucosa of WT and KO mice; bar length: 20 μm

Article Snippet: As in previous research [ ], paraffin-embedded Carnoy’s-fixed sections were hybridized with 10 ng/ml of a general bacterial 16S rRNA probe (EUB338, red, 5′-GCT GCC TCC CGT AGG AGT-3′, Boster) and immunostained for Mucin2 using the UEA-1 (Bauhinia bean lectin, FL-1061-2, Vector labs).

Techniques: Expressing, Methylation, Immunohistochemistry, Western Blot, Staining, Reverse Transcription Polymerase Chain Reaction, Immunofluorescence, Bacteria, Fluorescence, In Situ Hybridization